diff --git a/sanic/app.py b/sanic/app.py
index 3f48f1fd..e8cb5c17 100644
--- a/sanic/app.py
+++ b/sanic/app.py
@@ -1454,6 +1454,8 @@ class Sanic:
         asgi_app = await ASGIApp.create(self, scope, receive, send)
         await asgi_app()
 
+    _asgi_single_callable = True  # We conform to ASGI 3.0 single-callable
+
     # -------------------------------------------------------------------- #
     # Configuration
     # -------------------------------------------------------------------- #
diff --git a/sanic/asgi.py b/sanic/asgi.py
index 2a3c4540..8e13342c 100644
--- a/sanic/asgi.py
+++ b/sanic/asgi.py
@@ -312,13 +312,17 @@ class ASGIApp:
         callback = None if self.ws else self.stream_callback
         await handler(self.request, None, callback)
 
+    _asgi_single_callable = True  # We conform to ASGI 3.0 single-callable
+
     async def stream_callback(self, response: HTTPResponse) -> None:
         """
         Write the response.
         """
         headers: List[Tuple[bytes, bytes]] = []
         cookies: Dict[str, str] = {}
+        content_length: List[str] = []
         try:
+            content_length = response.headers.popall("content-length", [])
             cookies = {
                 v.key: v
                 for _, v in list(
@@ -351,10 +355,17 @@ class ASGIApp:
             ]
 
         response.asgi = True
-
-        if "content-length" not in response.headers and not isinstance(
-            response, StreamingHTTPResponse
-        ):
+        is_streaming = isinstance(response, StreamingHTTPResponse)
+        if is_streaming and getattr(response, "chunked", False):
+            # disable sanic chunking, this is done at the ASGI-server level
+            response.chunked = False
+            # content-length header is removed to signal to the ASGI-server
+            # to use automatic-chunking if it supports it
+        elif len(content_length) > 0:
+            headers += [
+                (b"content-length", str(content_length[0]).encode("latin-1"))
+            ]
+        elif not is_streaming:
             headers += [
                 (b"content-length", str(len(response.body)).encode("latin-1"))
             ]
diff --git a/sanic/response.py b/sanic/response.py
index 1f7b12de..9841bb2d 100644
--- a/sanic/response.py
+++ b/sanic/response.py
@@ -100,6 +100,8 @@ class StreamingHTTPResponse(BaseHTTPResponse):
         """
         data = self._encode_body(data)
 
+        # `chunked` will always be False in ASGI-mode, even if the underlying
+        # ASGI Transport implements Chunked transport. That does it itself.
         if self.chunked:
             await self.protocol.push_data(b"%x\r\n%b\r\n" % (len(data), data))
         else:
diff --git a/tests/test_response.py b/tests/test_response.py
index 6e2f2a9a..847246d3 100644
--- a/tests/test_response.py
+++ b/tests/test_response.py
@@ -238,7 +238,7 @@ def test_chunked_streaming_returns_correct_content(streaming_app):
 @pytest.mark.asyncio
 async def test_chunked_streaming_returns_correct_content_asgi(streaming_app):
     request, response = await streaming_app.asgi_client.get("/")
-    assert response.text == "4\r\nfoo,\r\n3\r\nbar\r\n0\r\n\r\n"
+    assert response.text == "foo,bar"
 
 
 def test_non_chunked_streaming_adds_correct_headers(non_chunked_streaming_app):