diff --git a/sanic/app.py b/sanic/app.py
index bb920c5c..8d846c3e 100644
--- a/sanic/app.py
+++ b/sanic/app.py
@@ -517,7 +517,7 @@ class Sanic:
     # -------------------------------------------------------------------- #
 
     def run(self, host="127.0.0.1", port=8000, debug=False, ssl=None,
-            sock=None, workers=1, loop=None, protocol=None,
+            sock=None, workers=1, protocol=None,
             backlog=100, stop_event=None, register_sys_signals=True,
             log_config=LOGGING):
         """Run the HTTP Server and listen until keyboard interrupt or term
@@ -531,7 +531,6 @@ class Sanic:
         :param sock: Socket for the server to accept connections from
         :param workers: Number of processes
                             received before it is respected
-        :param loop:
         :param backlog:
         :param stop_event:
         :param register_sys_signals:
@@ -550,7 +549,7 @@ class Sanic:
                           DeprecationWarning)
         server_settings = self._helper(
             host=host, port=port, debug=debug, ssl=ssl, sock=sock,
-            workers=workers, loop=loop, protocol=protocol, backlog=backlog,
+            workers=workers, protocol=protocol, backlog=backlog,
             register_sys_signals=register_sys_signals,
             has_log=log_config is not None)
 
@@ -577,7 +576,7 @@ class Sanic:
         return self
 
     async def create_server(self, host="127.0.0.1", port=8000, debug=False,
-                            ssl=None, sock=None, loop=None, protocol=None,
+                            ssl=None, sock=None, protocol=None,
                             backlog=100, stop_event=None,
                             log_config=LOGGING):
         """Asynchronous version of `run`.
@@ -597,7 +596,7 @@ class Sanic:
                           DeprecationWarning)
         server_settings = self._helper(
             host=host, port=port, debug=debug, ssl=ssl, sock=sock,
-            loop=loop or get_event_loop(), protocol=protocol,
+            loop=get_event_loop(), protocol=protocol,
             backlog=backlog, run_async=True,
             has_log=log_config is not None)
 
@@ -645,13 +644,6 @@ class Sanic:
                 warnings.simplefilter('default')
             warnings.warn("stop_event will be removed from future versions.",
                           DeprecationWarning)
-        if loop is not None:
-            if debug:
-                warnings.simplefilter('default')
-            warnings.warn("Passing a loop will be deprecated in version"
-                          " 0.4.0 https://github.com/channelcat/sanic/"
-                          "pull/335 has more information.",
-                          DeprecationWarning)
 
         self.error_handler.debug = debug
         self.debug = debug
diff --git a/sanic/server.py b/sanic/server.py
index 88ad339a..96b8e91c 100644
--- a/sanic/server.py
+++ b/sanic/server.py
@@ -1,7 +1,6 @@
 import asyncio
 import os
 import traceback
-import warnings
 from functools import partial
 from inspect import isawaitable
 from multiprocessing import Process
@@ -481,12 +480,6 @@ def serve_multiple(server_settings, workers):
     :param stop_event: if provided, is used as a stop signal
     :return:
     """
-    if server_settings.get('loop', None) is not None:
-        if server_settings.get('debug', False):
-            warnings.simplefilter('default')
-        warnings.warn("Passing a loop will be deprecated in version 0.4.0"
-                      " https://github.com/channelcat/sanic/pull/335"
-                      " has more information.", DeprecationWarning)
     server_settings['reuse_port'] = True
 
     # Handling when custom socket is not provided.